유원대학교 의약바이오학과

의약단백질공학 실험실

본 의약단백질 실험실에서는 인간 질병을 치료하기 위한 의약단백질의 개발 및 생산을 연구하고 있습니다. 생물화학공학과 생화학을 전공한 담당 교수는 당뇨병 치료 펩타이드 및 현전 용해제 등 다양한 의약단백질들을 연구해왔으며 학생들과의 공동 연구를 통하여 관련 대학원 진학과 제약회사로의 취업을 지도하고 있습니다.

연구 주제
1. 신규 당뇨병 치료용 펩타이드의 생물공학적 생산공정 개발
2. 재조합 항체의 활성형 생산 기술 개발
3. 방사능 피폭치료제 생산을 위한 재조합 대장균 개발
4. SsrA tagging system을 이용한 표적 단백질 분해조절 시스템 개발
5. D-ribose 생산을 위한 대사공학적으로 개량된 대장균 및 생물공학적 생산공정 개발  

실험실 연구 책임자: 김성건 교수

김성건 교수 약력

학력사항
2002-2008. 08. 공학박사 (석박사 통합과정), 서울대학교 협동과정 생물화학공학전공
박사 학위 논문 명: Engineering Analysis of Folding and Refolding of Industrial Proteins Expressed in Recombinant Escherichia coli
1996-2001. 02.  공학사, 영동대학교 유전공학과.

주요 경력
▷ 2010.03- 현재  : 조교수- 영동대학교 의생명과학과 
▷ 2007.07-2010.01: 박사후연구원- 미국 뉴저지의과대학교 (현 Rutgers University)
▷ 2007.03-2007.06: 선임연구원- 서울대학교, 농업생명과학연구원
▷ 2005.08-2005.10: 방문연구원- 미국 퍼듀대학교, 식품공학과 

논문
21. Y. J. Kim, D. H. Lee, C. W. Lee, Y. T. Rho, Y. J. Seong, Y. C. Park, D. Lee, and S. G. Kim, Production of D-ribose by metabolically engineered Escherichia coli, Bioprocess Biochemistry, Submitted.
20. J. C. Lee, B. J. Kong, J. Y. Shin, J. Shin, J. S. Oh, J. Y. Cho, K. Yoon, S. G. Kim, Y. H. Song, C. S. Shin, H. An, W. J. Lee, and D. H. Kweon, A pH-responsive High Density Lipoprotein-like Nanoparticle of Epothilone B. Biotechnology and Bioprocess Engineering, (2015), 249-258
19. N. Kang, E. Choi, S. G. Kim, and J. Hwang, Inhibitory effect of UvrD and DinG on the replication of ColE1-3 derived plasmids in Escherichia coli. Plasmid, (2015), 81, 32-41 (*co-corresponding author)
18. W. K. Min, S. G. Kim, J. H. Seo, Affinity maturation of single-chain variable fragment specific for aflatoxin B1 using yeast surface display. Food Chemistry, (2015) 188, 604-611
17. S. J. Park, S. H. Lee, K. J. Kim, S. G. Kim, H. Kim, H. Choe, S. Y. Lee, J. M. Yun, J. Y. Cho, J. Chun, K. S. Choi, Y. J. Son, Soluble Expression and Purification of Receptor Activator of Nuclear Factor-Kappa B Ligand Using Escherichia coli. Journal of Microbiology and Biotechnology, (2015) 25, 274-279
16. J. Y. Shin, Y. Yang, P. Heo, J. C. Lee, J. Y. Cho, K. Yoon, C. S. Shin, J. H. Seo, S. G. Kim, and D. H. Kweon, pH-responsive high-density lipoprotein-like nanoparticles to release paclitaxel at acidic pH in cancer chemotherapy. International Journal of Nanomedicine, (2012) 7, 2805-2816 (*co-corresponding author)
15. N. T. Hang, S. G. Kim and D. H. Kweon. Electrostatic immobilization of d-xylose isomerase to a cation exchanger for the conversion of d-xylose to d-xylulose. Korean Journal of Microbiology and Biotechnology, (2012) 40, 163-167 (*corresponding author)
14. S. G. Kim, W. K. Min, Y. T. Rho and J. H. Seo, Electrostatic interaction-induced inclusion body formation of glucagon-like peptide-1 fused with ubiquitin and cationic tag. Protein Expression and Purification, (2012) 84, 38-46
13. S. G. Kim, S. Y. Shin, Y. C. Park, C. S. Shin and J. H. Seo, Production and solid-phase refolding of human glucagon-like peptide-1 using recombinant Escherichia coli. Protein Expression and Purification, (2011) 78, 197-203
12. S.H. Kim, J. Y. Yun, S. G. Kim, J. H. Seo and J. B. Park. Production of xylitol from d-xylose and glucose with recombinant Corynebacterium glutamicum. Enzyme and Microbial Technology, (2010) 46, 366-371
11. L. Mao, Y. Tang, S. T. Vaiphei, T. Shimazu, S. G. Kim, R. Mani, E. Fakhoury, E. White, G. T. Montelione and M. Inouye. Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system. Journal of Structural and Functional Genomics, (2009), 10, 281-289
10. D. H. Lee, S. G. Kim, D. H. Kweon and J. H. Seo. Folding machineries displayed on a cation-exchanger for the concerted refolding of cysteine- or proline-rich proteins. BMC Biotechnology, (2009) 9
9. H. A. Yu, S. G. Kim, E. J. Kim, W. J. Lee, D. O. Kim, K. Park, Y. C. Park and J. H. Seo. Characterization of ubiquitin C-terminal hydrolase 1 (YUH1) from Saccharomyces cerevisiae expressed in recombinant Escherichia coli. Protein Expression and Purification, (2007) 56, 20-26
8. D. H. Lee, S. G. Kim, Y. C. Park, S. W. Nam, K. H. Lee and J. H. Seo, Proteome analysis of recombinant Escherichia coli producing human glucagon-like peptide-1. Journal of Chromatography B, (2007) 849, 323-330
7. S. G. Kim, J. A. Kim, H. A. Yu, D. H. Lee, D. H. Kweon, and J. H. Seo. Application of poly-arginine fused minichaperone to renaturation of cyclodextrin glycosyltransferase expressed in recombinant Escherichia coli. Enzyme and Microbial Technology, (2006) 39, 459-465
6. C. S. Rha, D. H. Lee, S. G. Kim, W. K. Min, S. G. Byun, D. H. Kweon, N. S. Han and J. H. Seo. Production of cyclodextrin by poly-lysine fused Bacillus macerans cyclodextrin glycosyltransferase immobilized on cation exchanger. Journal of Molecular Catalyst B, (2005) 34, 39-43.
5. S. G. Kim, D. H. Kweon, D. H. Lee, Y. C. Park and J. H. Seo. Coexpression of folding accessory proteins for production of active cyclodextrin glycosyltransferase of Bacillus macerans in recombinant Escherichia coli. Protein Expression and Purification, (2005) 41, 426-432.
4. D. H. Kweon, S. G. Kim, N. S. Han, K. M. Chung and J. H. Seo. Immobilization of cyclodextrin glycosyltransferase fused with poly-lysine using cation exchanger. Enzyme and Microbial Technology, (2005) 36, 571-578.
3. Y. C. Park, S. G. Kim, K. M. Park, K. H. Lee and J. H. Seo. Fed-batch production of d-ribose from sugar mixtures by transketolase deficient Bacillus subtilis SPK1. 2004. Applied Microbiology and Biotechnology, (2005) 66, 297-302.
2. D. H. Kweon, S. G. Kim and J. H. Seo. Purification and refolding of cyclodextrin glycosyltransferase expressed from recombinant Escherichia coli. Journal of Inclusion Phenomena and Macrocyclic Chemistry, (2004) 50, 37-41.
1. M. D. Kim, Y. S. Jeun, S. G. Kim and J. H. Seo. Comparison of xylitol production in recombinant Saccharomyces cerevisiae strains harboring XYL1 gene of Pichia stipitis and GRE3gene of S. cerevisiae. Enzyme and Microbial Technology, (2002) 31, 862-866.

특허
5. Korea Patent Filed No. 10-2014-0049730. Thymosin β4 for the production in E. coli
4. Korea Patent Filed No. 10-2014-0049729. Method for the production of thymosin β4 with yeast
3. Korea Patent Filed No. 10-2012-0059887. Method for production of ribose with metabolically engineered E. coli in medium with optimum ratio of xylose and glucose.
2. Korea Patent Filed No. 10-2011-0116023. Method for production of ribose with metabolically engineered Escherichia coli.
1. Korea Patent Filed No. 10-2002-057580. A biological method for producing d-ribose from d-xylose using a Bacillus subtilis mutant.